Biochemistry DMLT 3rd Semester HSBTE

Sample 1

Subject: Applied Clinical Biochemistry 221933 Time: 3 Hrs. M.M.: 60 3rd Sem / DMLT,

SECTION-A Note: Multiple choice questions. All questions are compulsory (6x1=6)

• Q.1 The bilirubin produced in the spleen and bone marrow diffuses into the blood, binds to _____and transported to the liver.

  a) Fibrinogen b) Potassium c) Albumin d) Globulin

• Q.2 SGOT is estimated by

  a) Evelyn & Malloy method b) DMSO method

  c) PNP method d) Reitman and Frankel's method

• Q.3 The enzyme amylase belongs to the class

  a) Hydrolase b) Isomerase c) Transferase d) Lyases

• Q.4 Maximum levels of acid phosphatase is found in which of the following cells

  a) Myelocytes b) T Lymphocytes c) B Lymphocytes d) Monocytes

• Q.5 Which of the following mechanisms contribute to a decrease in plasma calcium concentration?

  a) The action of vitamin D on the intestine b) The action of parathyroid hormone on the intestine

  c) The action of calcitonin on the kidney d) The action of parathyroid hormone on the kidney

• Q.6 HDL is called "good" cholesterol because

  a) It keeps cholesterol from building up in the lining of the arteries

  b) Your body makes more of it as you get older

  c) It helps keep your blood from clothing d) None of the above

  SECTION-B.

  Note: Objective/ Completion type questions. All questions are compulsory. (6x1=6)

• Q.7 Expand SGOT.

• Q.8 _______ is estimated by using dimethyl Sulphoxide method.

• Q.9 Define hypocalcemia.

• Q.10 Enlist any two methods for estimation of serum amylase.

• Q.11 Write the reference range of HDL and LDL cholesterol.

• Q.12 Name any two methods for estimation of serum cholesterol.

  SECTION-C

  Note: Short answer type questions. Attempt any eight questions out of ten questions. (8x4=32)

• Q.13 Schematically explain the formation of bilirubin.

• Q.14 Mention any four differences about direct and indirects bilirubin. Also write the reference values of direct and indirect bilirubin.

• Q.15 Describe the principle of Reitman and Frankel's method for SGOT estimation

• Q.16 Write the clinical significance of AST determination

• Q.17 Illustrate the procedure of serum amylase estimation.

• Q.18 Write the principle of ALP estimation.

• Q.19 Briefly explain the principle of serum calcium estimation.

• Q.20 Describe the procedure of serum potassium determination.

• Q.21 Mention the importance of various ratios of lipid profile. Q.22 Explain the principle of HDL estimation.

  SECTION-D

  Note: Long answer type questions. Attempt any two questions out of three questions. (2x8=16)

• Q.23 Describe the principle, procedure and clinical significance of serum bilirubin estimation.

• Q.24 Illustrate the principle, and procedure for estimation of serum glutamate-pyruvate transaminase.

• Q.25

  a) Write the principle of PNP method of acid phosphatase estimation.

  b) Schematically explain formation of cholesterol in brief.

Dr Pramila Singh

Sample 2

Sub.: Applied Clinical Biochemistry 221933 3rd Sem / Branch: DMLT Time: 3Hrs. M.M.: 60

SECTION-A Note: Multiple choice questions. All questions are compulsory (6x1=6)

• Q.1 The unconjugated bilirubin (non-polar form) is converted into a more polar form by conjugating with the help of

  a) Sulphuric acid b) Hydrochloric acid c) Glucuronic acid d) Acetic acid

• Q.2 SGPT is related to a) Heart b) Liver c) Lungs d) Pancreas

• Q.3 Amylase determination test is used for the detection of

  a) Hepatobiliary disease b) Peptic ulcers c) Cystic fibrosis d) None of these

• Q.4 Alkaline phosphate level is increased in

  a) Rickets b) Leukemia c) Cardial infection d) Pancreatic deficiency

• Q.5 Hypokalemia is defined as serum potassium less then

  a) 5.5 meg/L b) 4.5 meg/L c) 3.5 meg/L d) 2.5 meg/L

• Q.6 LDL cholesterol is called “bad” cholesterol as

  a) It causes low blood counts b) It changes heart rhythm

  c) It can cause blockage in the arteries d) Both a and B

  SECTION-B

  Note: Objective/Completion type questions. All questions are compulsory. (6x1=6)

• Q.7 Define bilirubin.

• Q.8 Name the transaminase enzyme which is used as biomarker of heart attack.

• Q.9 Normal value of serum amylase is _______.

• Q.10 Phosphorus is very importance for the normal functioning of heart. (True/False)

• Q.11 Write the reference range of triglycerides in normal human male adult.

• Q.12 Define lipoproteins.

  SECTION-C

  Note: Short answer type questions. Attempt any eight questions out of ten questions. (8x4=32)

• Q.13 Differentiate between conjugated and unconjugated bilirubin.

• Q.14 Describe the principle of Malloy & Evelyn method for serum bilirubin estimation.

• Q.15 Explain the clinical significance of ALT estimation.

• Q.16 Describe the procedure of SGPT determination.

• Q.17 Write the principle of serum amylase estimation.

• Q.18 Explain the principle of ACP estimation.

• Q.19 Mention the clinical significance of serum calcium determination.

• Q.20 Illustrate the procedure of serum potassium determination.

• Q.21 Briefly explain the formation of cholesterol.

• Q.22 Write the principle of LDL estimation.

  SECTION-D

  Note: Long answer type questions. Attempt any two questions out of three questions. (2x8=16)

• Q.23 Describe the formation and excretion of bilirubin in detail.

• Q.24 Explain the principle, procedure and clinical significance of SGOT estimation.

• Q.25

  a) Write the principle of triglycerides estimation.

  b) Illustrate the procedure of ALP estimation.

Dr Pramila Singh

Sample 3

Subject: Applied Clinical Biochemistry 221933 DMLT 3rd Semester, Time: 3 Hrs. M.M.: 60

SECTION-A Note: Multiple choice questions. All questions are compulsory (6x1=6)

• Q.1 LDL, HDL and VLDL belong to a) Lipid profile b) LFT c) RFT d) None

• Q.2 Normal value of serum bilirubin is a) 10mg/dl b) 100mg/dl c) 0.1mg/dl d) 1mg/dl

• Q.3 Idometric method is used to estimate______

  a) serum calcium b) inorganic Phosphorus c) serum amylase d) all of the above

• Q.4 Alfa amylase is released by_______ a) kidney b) brain c) liver d) salivary gland

• Q.5 Activity of ACP enzyme is maximum at pH. a) 4-5 b) 5-6 c) 8-9 d) 9-10

• Q.6 B100 protein is important component of lipoprotein a) VLDL b) LDL c) IDL d) All

  SECTION-B

  Note: Objective/ Completion type questions. All questions are compulsory. (6x1=6)

• Q.7 Write one clinical significance of calcium.

• Q.8 Write reference value of ACP.

• Q.9 Which lipoprotein is termed as bad cholesterol.

• Q.10 Expand SGPT.

• Q.11 Give the normal range of serum amylase.

• Q.12 Name the method for cholesterol estimation.

  SECTION-C

  Note: Short answer type questions. Attempt any eight questions out of ten questions. (8x4=32)

• Q.13 Differentiate between direct and indirect bilirubin.

• Q.14 Write down clinical significance of bilirubin estimation.

• Q.15 Differentiate between ALP and ACP.

• Q.16 Give the clinical importance of ACP estimation.

• Q.17 Explain principal SGPT and SGOT estimation.

• Q.18 Write down the normal range of lipid profile.

• Q.19 Write short note on metabolism of bilirubin.

• Q.20 Write short note on various isoenzymes of amylase.

• Q.21 What are conditions responsible for cholesterol elevation?

• Q.22 Expand LFT. List various tests involved in LFT.

  SECTION-D

  Note: Long answer type questions. Attempt any two questions out of three questions. (2x8=16)

• Q.23 Explain the formation and excretion of bilirubin.

• Q.24 Write down clinical importance of transferase and phosphatase group of enzymes present in body.

• Q.25 Explain procedure for estimation of various lipid profile components.

Dr Pramila Singh

Sample 4

3rd Sem. Branch: DMLT, Sub.: Applied Clinical Biochemistry Time: 3 Hrs. M.M.: 60

• SECTION-A Note: Multiple type Questions. All Questions are compulsory. (6x1=6)

• Q.1 OCPC is method for estimation of a) SGOT b) ALP c) Potassium d) Calcium

• Q.2 Normal value of serum amylase is a) 8-40 KU b) 60-180 CU c) 5-35 KU d) 6-20 CU

• Q.3 Which is not accelerator used in bilirubin estimation

  a) Methanol b) Dimethyl sulfoxide c) Caffeine benzoate d) Aminophenazone

• Q.4 Reference value of LDL: HDL ratio is less than a) 1.5:1 b) 2.5:1 c) 3.5:1 d) 4.5:1

• Q.5 Activity of ALP enzyme is maximum at pH. a) 4-5 b) 5-6 c) 8-9 d) 9-10 (1)

• Q.6 A1 protein is important component of lipoprotein a) HDL b) LDL c) IDL d) VLDL

  SECTION-B

  Note: Objective/Completion type questions. All questions are compulsory. (6x1=6)

• Q.7 Write one clinical significance of potassium.

• Q.8 Write reference value of ALP.

• Q.9 Define hypocalcaemia.

• Q.10 What is another name of ALT?

• Q.11 Give an example of transaminase group of enzymes.

• Q.12 At which pH ACP works.

  SECTION-C

  Note: Short answer type Questions. Attempt any eight questions out of ten Questions. (8x4=32)

• Q.13 Differentiate between conjugated and U conjugated bilirubin.

• Q.14 Write down clinical significance of calcium estimation.

• Q.15 Differentiate between HDL and LDL.

• Q.16 Write short note on cholesterol formation.

• Q.17 Explain principal of triglycerides estimation.

• Q.18 Write down normal range of ALP, ACP, SGPT, and SGOT

• Q.19 Write short note on metabolism of bilirubin.

• Q.20 Write short note on classification of amylase.

• Q.21 What are conditions responsible for jaundice.

• Q.22 Expand LFT. List various test involved in LFT and biomarker for liver.

  SECTION-D

  Note: Long answer questions. Attempt any two questions out of three Questions. (2x8=16)

• Q.23 Explain the clinical significance of serum bilirubin and serum amylase estimation.

• Q.24 Write down principal and procedures of SGPT and SGOT estimation.

• Q.25 Explain various components of lipid profile and their clinical significance.

Dr Pramila Singh