Biochemistry IVth Semester Question Bank HSBTE DMLT

Biochemistry IVth Semester Question Bank HSBTE DMLT. Urine Analysis, Renal Function Tests (Renal clearance Tests) RFT, Stool Chemistry, Electrophoresis and Chromatography, Thyroid function tests, and Automation in Biochemistry .

Dr. Pramila Singh

6/3/20246 min read

My post contentBiochemistry IVth Semester Question Bank HSBTE DMLT. Urine Analysis, Renal Function Tests (Renal clearance Tests) RFT, Stool Chemistry, Electrophoresis and Chromatography, Thyroid function tests, and Automation in Biochemistry .

Unit I

Urine Analysis: Normal composition of urine, Qualitative analysis of proteins, sugar, ketone bodies, bilirubin, and blood, Detailed discussion on glycosuria and albuminuria, Clinical significance of urine analysis.

SECTION A.

1. Normal pH range of urine is

i. 5.2 to 7.3, b) 3.1 – 9.6, c) 4.6 – 8, d) 2.3 – 4.5

2. When urine is excreted in excess amount is called___

i. Oligouria, b) Ketourea, c) Polyuria, d) none of the above

3. Ehrlich's test test is used to detect

a) urobilinogen, b) ketone bodies, c) bile pigment, d) glucose.

4. Where does the formation of bile pigment take place

a) Bone. b) blood, c)lungs, d) Liver

SECTION B

5. The test used to detect blood in urine_____
6. The test used to detect glucose in urine__________
7. Ketoneuria is a condition in which ketone bodies are present in urine (T/F)
8. Benzidine test is used to detect fecal fat (T/F)
9. Hematuria is a condition in which blood is present in urine (T/F)
a. Mention the normal range of albumin in urine
b. What are urinary proteins
10. Define glycosuria.
11. When glucose is present in urine is called____
12. Define haematuria Yellow-brown color of urine is due to the presence of------------Which test is used to find the presence of blood in urine ___
13. Yellow-brown color of urine is due to the presence of ___________.
14. What is urobilinogen
15. Milky appearance in urine is due to_________
16. What is hematuria Bendict test is done for_____ What is occult blood
17. Oligouria Define Oligouria
18. In 24 hours how much protein is excreted?

SECTION C

19. Give the procedure of benedict test for glucose estimation in urine
20. How we can detect ketone bodies in urine/ How to estimate ketone bodies in urine
21. . Give the clinical significance of glycosuria
22. Write the clinical importance of the urinary examination
23. Explain the principle and procedure of urinary protein estimation
24. Write a qualitative analysis of bile pigment.
25. Explain the sulphosalicylic acid test to detect proteins in urine./ Write the principle of urinary protein estimation.(IMP)
26. Write the preservation method of a urine sample(IMP).
27. Write chemical preservatives for urine specimens.
28. Write the composition of urine & clinical importance of urine Analysis.(IMP)
29. Write notes on albuminuria./ Explain albuminuria

SECTION D

30. Write the principle and procedure of urinary protein estimation
31. Explain the principle, procedure, and requirement of benedicts test in urine
32. What are ketone bodies? How to estimate ketone bodies in urine. OR
33. Qualitative analysis of ketone bodies.
34. Estimation of Bilirubin&Urinobilinogen in urine

Unit II

Renal Function Tests: Urea clearance Test. Creatinine clearance test, Clinical significance of Renal Function Test (RFT)

SECTION B

1. Normally, GFR is_______.
2. Write the normal values of urea clearance in human females.
3. Urinary creatinine test is done for diagnosis of renal diseases (T/F)
4. Reference value of urinary creatinine
5. In nephritic syndrome______is excreted in urine
6. Expand RFT.
7. Normal value of creatinine in urine is____
8. Give a normal range of urea in urine.
9. Creatinine is an anhydride of ______
10. The Berthlot reaction method is used to estimate ______
11. Creatinine reacts with picric acid _______
12. Urea is the major end product of _____ ammonia.
13. . Define urea.
14. Define creatinine clearance.
15. Write the principle of estimation of creatinine
16. Define creatinine clearance
17. Why creatinine Clearance is important.
18. Explain how urea clearance is calculated.
19. Write short notes on RFT
20. Write brief notes on the renal threshold.
21. Determine the formula for the urea clearance test.

SECTION C

23. Why creatinine clearance is important.
24. Explain how urea clearance is calculated.
25. Write short notes on RFT
26. Write brief notes on the renal threshold.
27. Determine the formula for the urea clearance test.

SECTION D

28. Explain the principle procedure for the Berthlot reaction method for urea estimation.
29. Calculate the urea clearance if the blood uria is 3 mg/dl, urine urea is 6 mg/dl, the amount of urine excreted per minute is 6 ml and the patient's surface area is 3.
30. Explain the creatinine clearance test's principle, procedure, and clinical significance.
31. Explain the principle, procedure and clinical significance of Urea Clearance test.

UNIT III

1. Clay color of stool due to_______?
a) bleeding in the lower gastrointestinal tract b) bleeding in the upper gastrointestinal tract c) post hepatic jaundice d) bleeding in the gastrointestinal tract
2. Define steatorrhoea.
3. Write the name of the test kit used for occult blood in the stool.
4. Which disease is diagnosed by the presence of occult blood?
5. What is the color of stool if occult blood is present?
6. Write a short note on Occult blood detection.
7. Write a note on gross examination of stool.
8. What are the organic constituents of urine in 24 hours?
9. Write a short note on Occult blood detection.
10. Write the chemical properties of stool
11. What are the organic constituents of urine in 24 hours?
a) Write the clinical importance of stool examination
b) Write the clinical significance of the detection of excess fat in stool.
12. Explain the physical characteristics & chemical composition of stool.
13. How to collect stool sample and also write the procedure for occult blood detection

UNIT IV

Electrophoresis and Chromatography: Principle and Theory of Electrophoresis, Types of Electrophoresis, Clinical significance/applications of Electrophoresis, Principle and Theory of Chromatography, Types of Chromatography, Clinical significance/applications of Chromatography

1. In which process mobile phase move over the stationary phase

a) chromatography b) electrophoresis, c) oncogenesis, d) none of above

2. Chromatography is used to determine the amount of drug present in the blood/urine sampled (T/F)
3. In the chromatography technique molecule separates on the basis of charge (T/F).
4. _______ is the technique that separates CHARGE PARTICLES using an electric field
5. Define chromatography
6. What is the mobile phase in electrophoresis. 5 marks
7. ______ To provide good separation of proteins in paper electrophoresis needs up to ____ hours
8. Application of electrophoresis 5 marks
9. Explain the principle theory application of paper chromatography.
10. Describe the principle procedure of cellulose electrophoresis
11. Difference between stationary and mobile phase.
12. Define Gel Electrophoresis.
13. Give the significance of chromatography.
14. Write the two procedures of Gel Electrophoresis.
15. Explain in detail the principle, procedure & clinical significance of paper chromatography. OR Explain the principle & procedure of paper chromatography.
16. What is the mobile phase in electrophoresis?
17. What is the principle of Gel Electrophoresis?
18. Write any 4 importance of electrophoresis.
19. Write the application of electrophoresis in biochemistry.
20. Name commonly used stationary phase in chromatography.
a) liquid, gas b) gas, solid c) solid, ice d) solid, liquid
21. At the isoelectric point net charge is equal to.........?
a) one b) zero c) both a & b d) two

Unit 5

Thyroid Function Tests and Automation in Biochemistry: Clinical Significance of T3, T4 and TSH, Anti-Thyroid Peroxidase (TPO) Test, Free Thyroid Profile Tests (Free T3, T4. and TSH Test), Definition and introduction of auto analyzers in clinical biochemistry, Classification and types of Auto analyzers.

1. What is Autoanalyzer?
2. Write another name for T3 and T4
3. What is automation in biochemistry? Explain its importance.
OR
Write a short note on automation in a clinical biochemistry laboratory
OR
Give in detail about the automation in biochemistry.
4. Write about the clinical significance of T3 & T4.
5. Write a short note on automation in the clinical biochemistry laboratory.
6. Write the normal range of TSH

a) 0.3-3.0 mU/I b) 0.4-4.0 mU/I c) 0.3-4.0 mU/I d) 0.4-3.0 mU/I

7. Expand TSH.
8. Automation needs additional training of staff about working and maintenance of machines. (T/F).
9. An enlarged thyroid gland is called_______
10. Write about discrete analysis and explain its components.
11. Write the normal range of T3
a) 100-200ng/dl b) 150-200ng/dl c) 150-300ng/dl d) 50-100ng/dl
12. In the auto analyzer the results are usually displayed in............
a) measuring unit b) reaction unit c) display unit d) photometer
13. Expand TFT.
14. Write the clinical importance of T3
15. Enlist the five advantages of automation in biochemistry.
16. Write the clinical significance of TSH, T3 and T4.
17. What are auto analyzers classify them.
18. Normal value of TSH is

a) 0.5-4.0ml U/L b) 1-4ml U/L c) 3.0-4.5ml U/L d) 2.5-6ml U/L

19. A condition in which the thyroid gland doesn't produce enough thyroid hormone

a) Hypothyroidism b) Thyroidism c) Hyperthyroidism d) None of the above

20. TSH stands for

a) Thyroid secreted hormone b) Thyroid standard hormone c) Thyroid-stimulating hormone d) None of the above.

21. Full form of TSH is____________.
22. Give the name of two auto analyzers.
23. Write a note on the thyroid function test.
24. Write a brief note on goiter.
25. An enlarged thyroid gland is called ________
26. Name the important thyroid hormones and their values.
27. Name any two hormones secreted by the thyroid gland.

Dr Pramila Singh