HSBTE Basic Microbiology ID: 221914 Semester: 1st DMLT Question Paper July 23

Semester: 1st Branch: DMLT

Subject Name: Basic Micro-Biology ID: 221914 Time Allowed: 3 Hrs. MM: 60

Section-A

Note: Multiple Choice questions. All questions are compulsory. 6x1=6

Q.1 Chocolate agar is an example of ----------

(a) Enriched Medium (b) Selective Medium

(c) Differential Medium (d) Nutrient Medium Ans: (a) Enriched Medium

Q.2 Rod-shaped bacteria are termed as

(a) Cocci (b) Bacilli (c) Spirochete (d) Spirillium Ans: (b) Bacilli

Q.3 Autoclaving inactivates/kills bacteria by

(a) Oxidation (b) Reduction (c) Denaturation (d) Hydrolysis Ans: (c) Denaturation

Q.4 Maximum growth of bacteria can be observed in - --------phase

(a) Log (b) Lag (c) Stationary (d) Decline Ans: (a) Log.

Q.5 Mycobacterium are best identified in

(a) Giemsa stain (b) Gram stain  (c) Fontana Stain (d) Acid-fast stain

Ans: (d) Acid-fast stain Note: Ziehl-Neelsen Stain is also known as Acid Fast Stain.

Q.6 Klintex papers are used as

(a) Bacteria-proof filter papers (b) To check the efficiency of sterilizers

(c) For aseptic monitoring (d) To check sterility testing.

Ans: b) To check the efficiency of sterilizers

Section-B

Note: Objective/Completion type questions. All questions are compulsory. 6x1=6

Q.7 Tell the functions of flagella in bacterial cell.

Ans: Bacterial Cell Locomotion.

Q.8 Define the term Thermal Death Time.

Ans: The duration of time required to kill a specific number of microbes at a specific temperature is called thermal death time.

Q.9 Tell why shorter exposure times are preferred in autoclaves to achieve sterilization.

Ans: Shorter exposure times allow for a more efficient sterilization process. Prolonged exposure to high temperatures can cause damage or alteration to certain materials.

Q.10 Distinguish between phototrophs and chemoautotrophs.

Ans: Phototrophs obtain energy from light. Chemoheterotrophs obtain energy from the chemical bonds of organic compounds (e.g., carbohydrates, fats, proteins).

Q.11 Give examples of chemical indicators used to evaluate the efficiency of sterilizers

Ans: Autoclave tape, which changes color or displays stripes when exposed to heat, is a common chemical indicator to evaluate the efficiency of sterilizers.

Q.12 Distinguish enriched media from nutrient broth

Ans: enriched media contain additional components to support the growth of a wider range of microorganisms including fastidious (nutritionally demanding) organisms that may not grow well on simple media. Nutrient broth is a basic and general-purpose medium that provides essential nutrients for the growth of a broad spectrum of microorganisms but may not meet the specific requirements of fastidious bacteria.

Section –C

Note: Short answer type Questions. Attempt any eight questions out of ten questions. 8x4= 32

Q.13 Review the nutritional requirements of bacteria for its growth and development.

Q.14 Write the informative notes on the streak plate method for the isolation of pure cultures.

Q.15 “Radiation sterilization is also referred to as cold sterilization”. Interpret the statement.

Ans: The term "cold sterilization" in this does not involve the application of heat to achieve sterilization. Radiation sterilization allows for the sterilization of heat-sensitive materials and does not require high temperatures. Thus radiation sterilization is also referred to as cold sterilization.

Q.16 Draw and show the different phases of the typical bacterial growth curve.

Q.17 Discuss the principle, working, and limitations of the Rideal Walker Co-efficient Test.

Q.18 List the four desirable properties to be possessed by an ideal disinfectant.

Q.19 Gram staining is also referred to as differential staining. Interpret the statement.

Q.20 Define and give an example of each (a) Selective media and (b) Enriched media.

Q.21 Handling of the Microscope is to be done with utmost care. Interpret the statement.

Q.22 Discuss the role of sterilization indicators.

Section-D

Note: Long answer questions. Attempt any two questions out of three questions.   2x8=16

Q.23 Discuss the working procedure of an autoclave with the help of a labeled sketch.

Q.24 Explain the different types of culture techniques to be utilized in microbiology along with their applications.

Q.25 How do different chemicals are useful in disinfection procedures? Explain with suitable examples.

Dr Pramila Singh