HSBTE DMLT: IMMUNOLOGY and MYCOLOGY Question Bank.

UNIT I

1.1 Introduction to Mycology

1.2 Characteristics and classification of medically important fungi

SECTION A

• 1. Candida albicans is a: a) Yeast b) Yeast fungi c) Moulds d) Dimorphic fungi

• 2. Fungi form a diverse group of: a) Motile eukaryotic b) Non motile eukaryotic c) Motile prokaryotic d) Non motile prokaryotic

• 3. The study of fungi is known as________ a) Bacteriology b) Parasitology c) Mycology d) Virology

• 4. Oral thrush is known as: a) Infection of the face b) Infection of the mucus membrane of the oral cavity c) Both a and b d) Infection of the nose and oral cavity.

• 5. Study of fungi is_______ a) Fungilogy b) Mycology c) Microbiology d) Virology

SECTION B

• 6. What is hypha

• 7. Name any two fungal infection

• 8. Define Mycology.

• 9. What is mycelium

• 10. Define Dermatophyte.

• 11. Define Endogenous Fungi.

• 12. What is fungi?

• 13. Define Candida.

• 14. Define Endogenous Fungi.

• 15. What is ringworm infection?

• 16. What are Moulds?

• 17. Name any two clinically important fungi(IMP)

SECTION C

• 18. Give general characteristics and lab diagnosis of dermatophytes

• 19. What is a fungus? Give its classification

• 20. Briefly describe the General Characteristics of fungi(IMP).

• 21. Classify the fungi as per Medical Importance.

• 22. Classify the fungi according to the industry's importance.

• 23. Briefly describe the medical importance of fungi.

• 24. Write a short note on the lab diagnosis of candida (IMP)

• 25. Which disease is caused by Candida fungi?

• 26. What are the general features of fungal colonies?

UNIT II

2.1 Collection, processing of samples for fungal infection in Skin, Nail, and Hair

• - KOH preparation

• - LCB (Lactophenol cotton blue)

• - India ink

• 2.2 Fungal Culture media

• SDA (sabouraud's dextrose agar) with and without antibiotics

• CMA (Com meal agar)

• BHI (Brain Heart Infusion)

SECTION A

• 1. Culture media commonly used for fungi is

• a) CMA b) SDA c) Corn meal agar d) Blood agar

SECTION B

• 2. Expand CMA?

• 3. Expand BHIA

• 4. Expand LCB

• 5. Give the name of culture media

• 6. How will you prepare 10 % KOH

• 7. Expand SDA

• 8. Expand KOH?

• 9. What is KOH

• 10. Agar and Broth.

• 11. SDA (sabouraud's dextrose agar) without antibiotics is used for________

• 12. SDA (Sabouraud’s dextrose agar) with antibiotics is used for_________.

• 13. Medically Importance of Brain Heart Infusion

• 14. Explain Inoculations of Culture Media.

SECTION C

• 15. Give the principle and procedure of LCB

• 16. Preparation of SDA fungal Culture Media OR Give the composition of SDA culture media (Imp

• 17. Collection of Skin specimens for fungal infection detection (IMP)OR

• 18. How will you collect skin samples for fungal identification

• 19. KOH Preparation for CSF specimen.

• 20. Preparation of CMA fungal Culture Media.

• 21. Medically Importance of Brain Heart Infusion Agar and Broth.

• 22. Collection of Nail specimens for Fungal infection detection.

• 23. India Ink Preparation for CSF specimen

• 24. Name commonly used culture media for fungal cultivation.

• 25. How will you isolate a fungus from a clinical sample?

SECTION D

• 26. Write down the principle and procedure of LCB test for identification of fungi.

UNIT III

3.1 Fungal Cultivation

Medically important fungi – Candida, Dermatophytes

3.2 Laboratory Contaminants – Penicillium, Rhizopus, Mucor, Aspergillus

• 1. How will you inoculate fungus in a clinical laboratory

• 2. Give the uses of penicillium

• 3. Cultivation of Dermatophyte.

• 4. Cultivation of Candida.

• 5. How will you inoculate fungus in the clinical lab?

• 6. Give general characteristics and Lab diagnosis of Aspergillus.

• 7. Give the general Technique for fungal cultivation.

UNIT IV

4.1 Antigen-Antibody Reactions

4.2 Principle and applications of agglutination, precipitation, and flocculation reactions

• 1. Blood group are determined by___________ reaction. a) Precipitation b) Gel diffusion c) Agglutination d) CFT

• 2. name of native or natural immunity. a) Innate immunity b) Acquired immunity c) Both a and b d) Humoral immunity.

• 3. How do conjunctivae calculate innate immunity? a) By Saliva secretions b) Through tears bacteria are washed away c) Tears are bactericidal and virucidal d) None of these.

• 4. ________Immunity is known as Vaccination. a) Artificial Passive immunity b) Natural active immunity c) Artificial innate immunity d) Natural innate immunity

• 5. The molecular weight complete antigen is________ a) 100000 daltons b) >10000 daltons c) <10000 daltons d) 11000-19000 daltons.

• 6. Explain co-agglutination test for Antigen-Antibody reaction.

• 7. Define Antibody.

• 8. What is Precipitation?

• 9. Define Agglutination with examples.

• 10. Define Antigen.

• 11. What is Agglutination?

• 12. Define precipitation with examples.

• 13. Name the two examples of antigen-antibody reactions.

• 14. Define Precipitation?

• 15. Give any two examples of Antigen-antibody reactions.

• 16. Give the Principle and Application of Precipitation.

UNIT V

5.1 Principle, techniques, and application of ELISA (direct and indirect)

5.2 Principle, techniques, and interpretation of i) Widal–Tube method/ Titre slide method, ii) Anti streptolysin O

iii) C-reactive protein VDRL/RPR, iv) VDRL/RPR, v) Rheumatoid factor (RF).

• 1. Syphilis is caused by a) Treponema pallidum b) Neisseria c) HIV d) HBV

• 2. Which type of test is done for enteric fever

a) Widal test b) ASO titer c) VDRL test d) Weil Felix test?

• 3. What is a serological test

• 4. Give the principal and clinical significance of the VDRL test.

• 5. How will you perform the CRP test

• 6. How will you perform ASO test

• 7. What is syphilis? How will you diagnose this disease

• 8. Describe the principle procedure and clinical importance of the Vidal test

• 9. What is ELISA? Mention the principle, procedure, and clinical importance of ELISA.

• 10. Widal test is done for________bacteria. a) Salmonella b) Parasite c) Virus d) Fungi

• 11. Expand ASO & RF.

• 12. Application and Result Interpretation of PRP Test.

• 13. Principle and Technique for RF.

• 14. Importance of ASO test.

• 15. Principle and importance of the Widal Test.

• 16. Principle of Direct ELISA.

• 17. Principle, application, and techniques of Indirect ELISA.

• 18. Expand VDRL & PRP.

• 19. Application and Result Interpretation of Widal Test.

• 20. Principle and technique for ASO.

• 21. Importance of CRP & RF test.

• 22. Principle and importance of the VDRL Test.

• 23. Principle of Indirect ELISA.

• 24. Principle, application, and techniques of Direct ELISA.

• 25. Expand ELISA

• 26. Write down the principle and procedure of the Widal Test.

• 27. Write down the principle and procedure of the VDRL test.

• 28. Write down the principle and procedure of the ELISA test.

• 29. List the names of enzymes used in the ELISA Test.

• 30. Name the causative agent of Enteric fever.

• 31. Give the Clinical Significance of the WIDAL Test.

• 32. Write a note on the VDRL Test.

• 33. Give the different types of ELISA & Explain them.

• 34. Describe the principle, procedure & interpretation of the RPR Test.

Dr Pramilka Singh